Primers Available
Organism:Chrysomya rufifacies mitochondrion
Repeat:(TTTA)3
Start:10082 End:10093
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCTTTATCATTAATCCCCAAAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCATTTGATGCAAGTGAGGT | |||||||||||||
| Tm_F_1 | 57.232 | Size_F_1 | 24 | Tm_R_1 | 58.215 | Size_R_1 | 21 | PRODUCT Size | 280 bp | Primer Start | 9838 | Primer End | 10117 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAACACCCATTAGCTATAGGACTAACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTCATTTGATGCAAGTGAGGT | |||||||||||||
| Tm_F_2 | 59.8 | Size_F_2 | 27 | Tm_R_2 | 59.615 | Size_R_2 | 22 | PRODUCT Size | 169 bp | Primer Start | 9950 | Primer End | 10118 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACACCCATTAGCTATAGGACTAACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTCATTTGATGCAAGTGAGGT | |||||||||||||
| Tm_F_3 | 58.717 | Size_F_3 | 26 | Tm_R_3 | 59.615 | Size_R_3 | 22 | PRODUCT Size | 168 bp | Primer Start | 9951 | Primer End | 10118 |